[02100575-CF] CAS 9003-98-9 | Deoxyribonuclease I, ≥2000 kunitz U/mg | MP bio
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Deoxyribonuclease I
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02100575-CF | CAS 9003-98-9 | DNase I, ≥2000 Kunitz U/mg | MPBIO
Key Features and Details
Deoxyribonuclease I
| SKU | 02100575-CF |
| Alternate Names | DNase I; Deoxyribonucleate 5′-oligonucleotido-hydrolase |
| Application Notes | DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from MP Biomedicals has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus. Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas. |
| Base Catalog Number | 100575 |
| Biochemical Physiological Actions | DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. |
| CAS # | 9003-98-9 |
| EC Number | 232-667-0 |
| Extinction Coefficient | E1%280= 11.1. |
| Formulation Details | A lyophilized powder containing a small amount of glycine-stabilizer |
| RTECS Number | RF0750000 |
| Source | Bovine Pancreas |
| Specific Activity | ≥2000 kunitz u/mg dw |
| Unit Definition | One unit causes an increase in absorbance at 260nm of 0.001/minute per ml at 25°C, pH 5.0, when acting on highly polymerized DNA in the presence of ionized magnesium and calcium. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
