[083209-CF] Zymolyase 100T (100KU/g) | MP bio
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083209-CF | Zymolyase 100T (100KU/g) | MPBIO
Key Features and Details
- Used to lyse living yeast cell walls to produce protoplast or spheroplast
- Can be used for glucan hydrolysis.
| SKU | 083209-CF |
| Application Notes | Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-1T has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration. |
| Base Catalog Number | 320931,320932 |
| Formulation Details | Lyophilized |
| pH | Optimum pH and temperature: pH 7.5, 35 °C for lysis of viable yeast cells; pH 6.5, 45 °C for hydrolysis of yeast glucan; pH Range: Stable through pH ranges 5-10 |
| Protein or Enzyme Type | Yeast Lytic Enzymes |
| Source | Arthrobacter luteus |
| Specific Activity | minimum of 100,000 units/g |
| Specificity | The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya; Candida; Debaryomyces; Eremothecium; Endomyces; Hansenula; Hanseniaspora; Kloekera; Kluyveromyces; Lipomyces; Metschikowia; Pichia; Pullularia; Torulopsis; Saccharomyces; Saccharomcopsis; Saccharomycodes; Schwannimomyces and others. |
| Unit Definition | The amount of activity causing a decrease in absorbance at 800 nm of 30%. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
