[112069400] RPM Yeast Plasmid Isolation Kit, 100 preps | MP bio
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Brand: MP Bio
For the isolation of plasmid DNA from yeast cells. Uses the GLASSMILK purification process in conjunction with SPIN modules.
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112069400 | RPM Yeast Plasmid Isolation Kit, 100 preps | MP Bio
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Key features and details
For the isolation of plasmid DNA from yeast cells. Uses the GLASSMILK purification process in conjunction with SPIN modules.
| SKU | 112069400 |
| Alternate Names | Yeast Plasmid Miniprep Purification Kit |
| Application Notes | The Yeast Plasmid Isolation Kit is a Rapid Pure Miniprep Kit designed for yeast cells. The plasmid isolated from yeast is suitable for PCR and bacterial transformation. The same kit can be used to isolate plasmid from bacterial transformants for use in any downstream application (PCR, sequencing, restriction enzyme digestion, labeling, etc). Plasmid DNA mini-preps from yeast. Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen. |
| Base Catalog Number | 2069400 |
| Components | Yeast Lysing Matrix |Alkaline Lysis Solution |Neutralizing Solution |GLASSMILK™ Spin Buffer |Wash Solution Concentrate |SPIN Filters |Catch Tubes |Pre-Lysis Buffer |
| Format | Geneclean® GLASSMILK™ slurry plus SPIN filter |
| Pack Size | 100 Preps |
| Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| pH | Alkaline Lysis Solution pH Range: 12.6-12.8 |Neutralizing Solution pH Range: 5.5-5.9 |Wash Solution Concentrate pH Range: 7.4-7.7 |Pre-Lysis Buffer pH Range: 6.6-7.0 |
| Preparation Method | Before first use, add the 56 mL of 200 proof (100% ethanol) to the Wash Solution Concentrate (56 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C. |
| Safety Symbol | GHS07 |GHS05 |
| Sample Source | Yeast and Fungi |
| Sample Type | Yeast Plasmid Prep |
| Sample Volume | 1.5 ml of yeast culture |
| Unit Definition | Yields up to 10 μg of plasmid DNA Per 1.5 ml overnight culture typical |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| brand | MP Bio |
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