[02101168-CF] CAS 606-68-8 | β-Nicotinamide adenine dinucleotide reduced, disodium salt | MP bio
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β-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt
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02101168-CF | CAS 606-68-8 | β-NADH, disodium salt, 100mg (MPBIO) | MPBIO
Key Features and Details
β-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt
| SKU | 02101168-CF |
| Alternate Names | β-DPNH; β-NADH, 2Na; DPNH; Diphosphopyridine nucleotide reduced form; NADH ; Reduced DPN; Cozymase, reduced form; Dihydrophosphopyridine nucleotide, reduced form; Dihydronicotinamide adenine dinucleotide |
| Application Notes | β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases). Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent; whereas, β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10−7 M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported. |
| Base Catalog Number | 101168 |
| Biochemical Physiological Actions | NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, beta-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations. |
| CAS # | 606-68-8 |
| EC Number | 210-123-3 |
| Extinction Coefficient | EmM = 6.22 (340 nm) and 14.4 (259 nm, pH 9.5)(Lit.) |
| Format | Powder |
| Molecular Formula | C21H27N7O14P2Na2 |
| Molecular Weight | 709.409 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Purity | ≥98% |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | Water: λ max: 258 – 260 nm |
