[02150704-CF] CAS 9001-12-1 | Collagenase type 3, >100 U/mg | MP bio
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Brand: MP Bio
Clostridiopeptidase A; EC 3.4.24.3 /
Collagenase Type 3
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02150704-CF | CAS 9001-12-1 | Collagenase type 3, >100 U/mg | MP bio
관련 문서 다운로드 (MSDS, COA, SPEC, Datasheet, Brochures, Manuals) :
Key features and details
Collagenase Type 3
| SKU | 02150704-CF |
| Alternate Names | Clostridiopeptidase A; EC 3.4.24.3 |
| Application Notes | Collagenase has found widespread application in the isolation of specific cell types from attendant connective tissue. Most preparations contain collagenase, non-specifc protease, and clostripain activities. These enzymes act synergistically to degrade collagen and other intercellular material. Crude Collagenases are suitable for isolation of cells from many types of animal tissue. The wide spectrum of activity of crude collagenase makes it especially useful as a debriding agent. |
| Base Catalog Number | 150704 |
| Biochemical Physiological Actions | Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) – N, N, N’,N’-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. |
| CAS # | 9001-12-1 |
| EC Number | 232-582-9 |
| Format | Powder |
| Hazard Statements | H315-H319-H334-H335 |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| Safety Symbol | GHS07, GHS08 |
| Source | Clostridium histolyticum |
| Specific Activity | >100 u/mg |
| Typical Working Concentration | For typical assay: Dissolve enzyme at a concentration of 1 mg/mL in 0.05 M TES with 0.36 mM calcium chloride, pH 7.5. Dilutions run are 1/10 and 1/20 in the above buffer. |
| Unit Definition | One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| brand | MP Bio |
|---|---|
| Pakage | 1x1g, 1x5g |
