[02150902-CF] CAS 1609-47-8 | Diethyl pyrocarbonate | MP bio
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02150902-CF | CAS 1609-47-8 | Diethyl pyrocarbonate | MPBIO
Key Features and Details
Diethyl Pyrocarbonate
| SKU | 02150902-CF |
| Alternate Names | Diethyl Oxydiformate; Dicarbonic Acid Diethyl Ester; DEPC; Ethoxyformic acid anhydride; Diethyl dicarbonate; DEP; Pyrocarbonic acid diethyl ester; Oxydiformic acid diethyl ester |
| Application Notes | DEPC is effective as a nuclease inhibitor.Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl Pyrocarbonate is used to modify proteins and nucleic acids. Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms. |
| Base Catalog Number | 150902 |
| Beilstein Registry Number | 637031 |
| Biochemical Physiological Actions | Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation. It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles.. |
| CAS # | 1609-47-8 |
| EC Number | 216-542-8 |
| Hazard Statements | H302-H315-H319-H335 |
| Melting Point | < 25 °C |
| Molecular Formula | C6H10O5 |
| Molecular Weight | 162.141 g/mol |
| Personal Protective Equipment | Eyeshields, Faceshields, full-face respirator , Gloves, multi-purpose combination respirator cartridge respirator filter |
| Refractive Index | n20/D 1.398(lit.) |
| RTECS Number | LQ9350000 |
| Safety Symbol | GHS07 |
| Typical Working Concentration | For Treatment of water to inhibit RNase: Add 1 ml DEPC to 1 liter of water. Let sit overnight. Autoclave. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
