[02190327-CF] CAS 64-72-2 | Chlortetracycline hydrochloride, >900 Units/mg | MP bio
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02190327-CF | CAS 64-72-2 | Chlortetracycline hydrochloride, >900 Units/mg | MPBIO
Key Features and Details
- Cell-permeable fluorescent probe for measuring calcium flux in endoplasmic reticulum and other intracellular stores in situ
| SKU | 02190327-CF |
| Alternate Names | Aureomycin; Chlorotetracycline hydrochloride; 7-Chlorotetracycline hydrochloride; aureocycline; auxeomycin; biomycin; 7-chlorotetracycline |
| Application Notes | Cell-permeable fluorescent probe for Ca2+ for measuring calcium flux in endoplasmic reticulum and other intracellular stores in situ. |
| Base Catalog Number | 190327 |
| Beilstein Registry Number | 3858364 |
| Biochemical Physiological Actions | Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-positive. Less active against Gram-negative bacterial than tetracycline. Mode of Resistance: Loss of cell wall permeability. |
| CAS # | 64-72-2 |
| EC Number | 200-591-7 |
| Format | Powder |
| Hazard Statements | H315-H319-H335 |
| Molecular Formula | C22H23ClN2O8 • HCl |
| Molecular Weight | 515.34 g/mol |
| Personal Protective Equipment | Dust mask , Eyeshields, Gloves |
| pKa | 3.3, 7.4 and 9.3 at 25 °C.(Lit.) |
| Preparation Method | To prepare Stock Solutions: Add the appropriate amount of powder to water while mixing (not the reverse), as adding water to the powders may result in insoluble mixtures that cannot be filtered without losing potency. If desired, the pH may be adjusted, but a basal media with greater than 2 g/L sodium bicarbonate can easily absorb up to 2 % of an acidic antibiotic solution; therefore, an antibiotic solution with an acidic pH would not influence the pH of the media. After ensuring that the resultant solution is transparent, sterile filter and store in small aliquots at appropriate temperature until use. Prior to use of the media, aseptically add the appropriate amount of antibiotic to the basal media in order to obtain the required working concentration. Mix well and sterile filter if asepsis is believed to have been compromised. |
| RTECS Number | QI7800000 |
| Safety Symbol | GHS07 |
| Specific Activity | >900 u/mg |
| Sterilization of Solutions | Solutions should be filter sterilized. Store in small aliquots at appropriate temperature until use. |
| Typical Working Concentration | For Gram Positive Bacteria & Gram Negative Bacteria : 10 mg/L |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
