[02195283-CF] CAS 11028-71-0 | Concanavalin A, highly purified | MP bio
02195283-CF | CAS 11028-71-0 | Concanavalin A, highly purified | MPBIO
Key Features and Details
Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5.0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25,500. Con-A does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding. Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is usually inhibited by a simple monosaccharide, but for some lectins, di, tri, and even polysaccharides are required.
| SKU | 02195283-CF |
| Alternate Names | Con A |
| Application Notes | Lectin are used in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Con-A has been used to elucidate structural changes in the membrane surface of transformed cells. Con-A conjugates of antitumor drugs have been used in drug delivery systems in cultured cells. |
| Base Catalog Number | 195283 |
| Biochemical Physiological Actions | Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6. Con-A binds specifically to mannosyl and glucosyl residues of polysaccharides and glycoproteins. Unmodified hydroxyl groups at the C3, C4 and C6 positions of D-glucopyranosyl or D-mannopyranosyl rings may be essential for binding. Each subunit of Con-A contains one calcium ion and one manganese ion. Removal of these cations by dialysis under acidic conditions abolishes the carbohydrate-binding activity. |
| CAS # | 11028-71-0 |
| EC Number | 234-258-2 |
| Extinction Coefficient | 280nm = 11.4 (0.1 M NaCl)(Lit.) |
| Format | Powder, Essentially Salt Free, Highly Purified |
| Grade | Higly Purified |
| Hazard Statements | H317-H334-H361 |
| Isoelectric Point | 4.5, 4.7, 5.0-5.1, and 5.4-5.5 (several isoforms)(Lit.) |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| RTECS Number | GK6890000 |
| Safety Symbol | GHS08 |
| Solubility | Soluble in water (10 mg/ mL-slightly hazy, colorless solution)(40 mg/mL in 0.01 M PBS, pH 6.8 containing 0.1 mM Ca2+, 0.1 mM Mn2+). |
| Source | Canavalia ensiformis seeds (jack beans) |
| Specificity | Carbohydrate Specificity: a-Mannose; a-Glucose; Branched Mannose. |
| Typical Working Concentration | Reconstitution: Reconstitute with buffer to a final concentration of 1 mg/mL. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
