[02195303-CF] CAS 9001-63-2 | Lysozyme type VI, molecular biology reagent, ≥23,500 Shugar units/mg | MP bio
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02195303-CF | CAS 9001-63-2 | Lysozyme VI, MB reagent, ≥23,500 Shugar U/mg | MPBIO
Key Features and Details
Lysozyme Type Vi
| SKU | 02195303-CF |
| Alternate Names | Muramidase; Mucopeptide N-acetylmuramoylhydrolase |
| Application Notes | Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. |
| Base Catalog Number | 195303 |
| Biochemical Physiological Actions | Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M). |
| CAS # | 9001-63-2 |
| EC Number | 232-620-4 |
| Extinction Coefficient | 26.4 (280 nm) (Lit.) |
| Format | lyophilized, Salt-free, 3X Crystallized, Albumin-Free, Type VI |
| Grade | Molecular Biology Grade |
| Isoelectric Point | pH 11.0 (lit) |
| Molecular Weight | 2235.597 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| RTECS Number | OL5987000 |
| Source | Chicken eggwhite |
| Specific Activity | ≥23,500 Shugar units/mg |
| Unit Definition | One unit will produce a decrease in A450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
