[02195367-CF] CAS 9001-75-6 | Pepsin, >2,000 U/mg protein | MP bio
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02195367-CF | CAS 9001-75-6 | Pepsin, >2,000 U/mg protein | MPBIO
Key Features and Details
- Acid protease used to digest proteins
- Commonly used in the preparation of Fab fragments from antibodies
- Contains the “cathepsin” component which has milk curdling activity
- Broad range of substrate activity and demonstrates an esterase activity
| SKU | 02195367-CF |
| Alternate Names | Pepsin A |
| Application Notes | Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. |
| Base Catalog Number | 195367 |
| Biochemical Physiological Actions | Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin.Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages. |
| CAS # | 9001-75-6 |
| EC Number | 232-629-3 |
| Extinction Coefficient | E1%280 = 14.7 |
| Format | Lyophilized powder |
| Hazard Statements | H315-H319-H334-H335 |
| Isoelectric Point | pH 1.0 |
| Personal Protective Equipment | dust mask, Eyeshields, Faceshields, Gloves |
| Protein or Enzyme Type | Proteins, Enzymes & Peptides |
| RTECS Number | SC6132000 |
| Safety Symbol | GHS07, GHS08 |
| Source | Porcine Stomach Mucosa |
| Specific Activity | >2,000 u/mg protein |
| Unit Definition | One unit will produce a delta A280 of 0.001 per minute at pH 2.0 and 37°C, measured as TCA-soluble products using hemoglobin as substrate |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
