[04802511] CAS 1239-45-8 | Ethidium bromide solution, 10 mL | MP bio
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04802511 | CAS 1239-45-8 | Ethidium bromide solution | MPBIO
Key Features and Details
Ethidium Bromide Solution
| SKU | 04802511 |
| Alternate Names | 3,8-Diamino-5-ethyl-6-phenylphenanthridiniumbromide (6CI); Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide(8CI,9CI); 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide; 2,7-Diamino-9-phenylphenanthridine ethobromide; Dromilac; Homidium bromide |
| Application Notes | Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. |
| Base Catalog Number | 802511 |
| Beilstein Registry Number | 3642536 |
| Biochemical Physiological Actions | Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. |
| CAS # | 1239-45-8 |
| Density | 0.34 g/cm³ |
| EC Number | 214-984-6 |
| Flash Point | >100 °C |
| Format | Solution |
| Melting Point | 260-262 deg C (decomposes) |
| Molecular Formula | C21H20BrN3 |
| Molecular Weight | 394.316 g/mol |
| Pack Size | 10 mL |
| RTECS Number | Ethidium Bromide SF7950000 |
| Solubility | Solubility in <a class=”pubchem-internal-link CID-8019″ href=”https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol”>2-methoxyethanol</a> is 20 mg/mL; <a class=”pubchem-internal-link CID-702″ href=”https://pubchem.ncbi.nlm.nih.gov/compound/ethanol”>ethanol</a>, 2 mg/mL |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Pressure | 1.2X10-12 mm Hg at 25 deg C /Estimated/ |
