[091689649] CAS 9002-07-7 (trypsin) | 0.25% Trypsin-EDTA (1X), 100 mL | MP bio
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091689649 | CAS 9002-07-7 (trypsin) | 0.25% Trypsin-EDTA (1X) | MPBIO
Key Features and Details
0.25% Trypsin and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL
| SKU | 091689649 |
| Alternate Names | Trypsin (1X), Trypsin-EDTA solution |
| Base Catalog Number | 1689649 |
| CAS # | 9002-07-7 (trypsin) |
| Concentration | 0.25% (w/v) solution | 0.25% Trypsin (1:250) and 1mM EDTA In Modified HBSS Without Ca++ and Mg++ |
| Format | Solution |
| Formulation Details | Liquid | 0.25% Trypsin (1:250) and 1mM EDTA In Modified Hbss Without Ca++ and Mg++ |
| Media Type | Mammalian Media |
| Molecular Weight | Approximately 23;000 (trypsin) |
| Pack Size | 100 mL |
| pH | 7.0 – 7.3 |
| Source | Porcine (for trypsin) |
| Specific Activity | 1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units |
| Specificity | Specificity: The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. Forms complexes with α2-macroglobulin. Can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds. |
| Sterility | Sterile |
| Unit Definition | One BAEE unit will produce a ΔA253 of 0.001 per minute at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path). One TAME unit hydrolyzes 1 μmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25 °C, pH 8.2, in the presence of 0.001 M calcium ion. One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
