[113037012-CF] M9 minimal medium salts | MP bio
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Brand: MP Bio
M9 Minimal Media /
M9 Minimal Salts are used for preparing a defined synthetic medium for the growth of E. coli host.
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113037012-CF | M9 minimal medium salts | MP bio
관련 문서 다운로드 (MSDS, COA, SPEC, Datasheet, Brochures, Manuals) :
Key features and details
M9 Minimal Salts are used for preparing a defined synthetic medium for the growth of E. coli host.
| SKU | 113037012-CF |
| Alternate Names | M9 Minimal Media |
| Application Notes | M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation. |
| Base Catalog Number | 3037012 |
| Components | Contents/L of 5x solution: 33.9 g Na2HPO4 15 g KH2PO4 2.5 g NaCl, 5 g NH4Cl |
| Format | Powder |
| Formulation Details | 33.9 g Na2HPO4; 15 g KH2PO4; 2.5 g NaCl; 5 g NH4Cl = 56.4 g/L of a 5x solution. |
| Grade | Molecular Biology Certified™ |
| Media Type | M9 Minimal Medium |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| pH | Acceptable pH range is: 6.5 – 7.2 |
| Preparation Method | Media Preparation:Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in M9 Minimal Medium Salt formulations. Chosen reagents are combined in the proper proportions according to the formulation listed in the Certificate of Analysis and thoroughly blended for a uniform distribution. |
| Solubility | Soluble in water |
| Sterilization of Solutions | A 56.4 g sample of M9 Minimal Salts was dissolved in water to 1 liter, the pH adjusted to 7.4, if needed, and the solution autoclaved to make a sterile 5X concentrate. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| brand | MP Bio |
|---|---|
| Pakage | 1x1kg, 1x227g, 1x454g |
