[114063102-CF] CAS 7240-90-6 | X-GAL, powder | MP bio
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Brand: MP Bio
5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG /
X-Gal (5-Bromo-4-Chloro-3-Indolyl-ß-D-galactopyranoside) is a β-galactosidase substrate. In the presence of β-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate.
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114063102-CF | CAS 7240-90-6 | X-GAL, powder | MP bio
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Key features and details
X-Gal (5-Bromo-4-Chloro-3-Indolyl-ß-D-galactopyranoside) is a β-galactosidase substrate. In the presence of β-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate.
| SKU | 114063102-CF |
| Alternate Names | 5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG |
| Application Notes | X-Gal is used for the identification and selection of galactosidase producing bacteria (bacteria Lac+). X-Gal is particularly useful in molecular biology applications to detect the activity of β-galactosidase which is frequently used as a reporter gene. In cloning, X-Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Lac? bacterial colonies resulting from α-complementation will appear blue whereas bacterial colonies containing plasmid with DNA inserted in the lacZ region, will be incapable of α-complementation and will appear white. Many other applications also use X-Gal as a substrate to detect β-galactosidase activity, which include β-galactosidase-antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction, and the detection of micrometastasis formation during tumor progession. |
| Base Catalog Number | 4063102 |
| Beilstein Registry Number | 1402009 |
| CAS # | 7240-90-6 |
| EC Number | 230-640-8 |
| Format | Powder |
| Grade | Ultra Pure |
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight | 408.6 |
| Optical Rotation | -61.9%(c=1, DMF_water=1:1, Lit.) |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice |
| Purity | (TLC) >99% |
| Solubility | Soluble in dimethylformamide (2% in DMF), Dimethyl sulfoxide (20 mg/mL) & DMF:MeOH (1:1) (50 mg/mL). |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| brand | MP Bio |
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