[114520512-CF] CSM-TRP-URA, powder | MP bio
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Brand: MP Bio
Complete Supplement Mixture /
Double dropout formulation (without Tryptophan and Uracil) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.
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114520512-CF | CSM-TRP-URA, powder | MP bio
관련 문서 다운로드 (MSDS, COA, SPEC, Datasheet, Brochures, Manuals) :
Key features and details
Double dropout formulation (without Tryptophan and Uracil) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.
| SKU | 114520512-CF |
| Alternate Names | Complete Supplement Mixture |
| Application Notes | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
| Base Catalog Number | 4520512 |
| Format | Powder |
| Formulation Details | Contents per Liter: Adenine 10* mg |L-Arginine HCl 50 mg |L-Aspartic Acid 80 mg |L-Histidine HCl 20 mg |L-Isoleucine 50 mg |L-Leucine 100 mg |L-Lysine HCl 50mg |L-Methionine 20** mg |L-Phenylalanine 50 mg |L-Threonine 100** mg |L-Tryptophan 0 mg |Uracil 0 mg| L-Tyrosine 50 mg |L-Valine 140 mg |Total 720.000 |*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. CSM formulations are available that contain 20 or 40 mg/liter adenine. **80mg/liter of DL-Homoserine is substituted for L-Threonine in mixtures where L-Methionine is dropped out. |
| Grade | Molecular Biology Certified™ |
| Media Type | Yeast CSM |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice |
| pH | pH Range (with DOB, after autoclave): 4.0 – 4.8 at 25 °C |
| Preparation Method | Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM or CSM-Dropout formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7g DOBA powder and 0.79g of CSM or the appropriate weight of CSM-Dropout to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured. |
| Solubility | Soluble in water. |
| Sterilization of Solutions | Autoclave at 121 °C for 15 minutes |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| brand | MP Bio |
|---|---|
| Pakage | 1x100g, 1x10g |
