[114842065-CF] SD medium-ADE-HIS-LEU-TRP (1 L pouches) | MP bio
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Brand: MP Bio
Synthetic defined medium without ADE-HIS-LEU-TRP /
Dropout formulation of synthetic defined (SD) yeast medium (without Adenine, Histidine, Leucine and Tryptophan) includes all necessary components for selective growth of Saccharomyces cerevisiae. Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.
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114842065-CF | SD medium-ADE-HIS-LEU-TRP (1 L pouches) | MP bio
관련 문서 다운로드 (MSDS, COA, SPEC, Datasheet, Brochures, Manuals) :
Key features and details
Dropout formulation of synthetic defined (SD) yeast medium (without Adenine, Histidine, Leucine and Tryptophan) includes all necessary components for selective growth of Saccharomyces cerevisiae. Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.
| SKU | 114842065-CF |
| Alternate Names | Synthetic defined medium without ADE-HIS-LEU-TRP |
| Application Notes | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
| Base Catalog Number | 4842065 |
| Format | Pouch |
| Formulation Details | Contents per Liter: 1.7 g YNB |5 g Ammonium Sulfate |20 g Dextrose |0.61 g CSM-Ade-His-Leu-Trp |
| Grade | Molecular Biology Certified™ |
| Media Type | Yeast Media |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice |
| pH | pH Range (with DOB, after autoclave): 4.0 – 4.8 at 25 °C |
| Preparation Method | Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the Synthetic Defined (SD) or Synthetic Defined Agar (SDA) or SD/SDA-Dropout formula. SD is 1.7 g Yeast Nitrogen Base, 5 g Ammonium Sulfate and 20g Dextrose per liter. SDA is SD with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium by adding 27.49 SD powder or the appropriate weight ofSD-Dropout to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. |
| Solubility | Soluble in water. |
| Sterilization of Solutions | Autoclave at 121 °C for 15 minutes. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| brand | MP Bio |
|---|---|
| Pakage | 1×100.5LPouches, 1x101LPouches |
